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Development of Novel Barley with Improved Beer Foam and Flavor Stability�The Impact of Lipoxygenase-1-Less Barley in the Brewing Industry

MBAA TQ vol. 43, no. 2, 2006, pp. 131-135  |  VIEW ARTICLE

Naohiko Hirota (1), Hisao Kuroda (2), Kiyoshi Takoi (3), Takafumi Kaneko (1), Hirotaka Kaneda (2), Ikuya Yoshida (3), Masachika Takashio (2), Kazutoshi Ito (1), and Kazuyoshi Takeda (4). 1. Bioresources Research and Development Laboratories, Sapporo Breweries Ltd., 37-1 Kizaki-Nitta, Ota, Gunma 370-0393, Japan. 2. Frontier Laboratories of Value Creation, Sapporo Breweries Ltd., 10 Okatome, Shizuoka 425-0013, Japan. 3. Production & Technology Development Center, Sapporo Breweries Ltd., 10 Okatome, Shizuoka 425-0013, Japan. 4. Research Institute for Bioresources, Okayama University, 2-20-1 Chuo, Kurashiki 710-0046, Japan.

Abstract
The purpose of this study is to understand the possibility of using lipoxygenase-1-less (LOX-less) barley in brewing and to establish an effective breeding system to create advanced LOX-less varieties. Brewing trials indicate that LOX-less barley can improve the foam stability and flavor stability of beer. To establish an effective barley breeding system, we developed a DNA marker for the LOX-less trait based on the molecular basis of the deficiency. This direct marker is universally applicable to the selection of the LOX-less trait in any practical crosses. We are incorporating the LOX-less trait into several advanced barley varieties worldwide in our network breeding system.

Keywords: barley, breeding, flavor stability, foam stability, lipoxygenase-1, LOX-less

 

S�ntesis
Se propuso con este estudio investigar la posibilidad de usar �lipoxygenasa-1-less (LOX-less)� cebada para la elaboraci�n de cerveza, como tambi�n establecer un sistema eficaz de cultivaci�n para crear variedades avanzadas tipo �LOX-less�. Pruebas cerveceras indican que cebada �LOX-less� puede mejorar la estabilidad sensorial y la estabilidad de la espuma de la cerveza. Con el fin de establecer un sistema eficaz de cultivaci�n o creaci�n de diferentes variedades con esta caracter�stica, se desarroll� un marcador ADN para la caracter�stica �LOX-less�, fundamentado sobre la base molecular de la deficiencia. Este marcador directo se puede aplicar universalmente a la selecci�n de la caracter�stica �LOX-less� en cualquier cruce pr�ctico. Estamos incorporando la caracter�stica �LOX-less� en varias de nuestras variedades avanzadas de cebada en todo el mundo, en nuestra red de creaci�n de variedades.

Palabras claves: cebada, creaci�n de variedades, estabilidad sensorial, estabilidad de espuma, lipoxygenase-1, LOX-less

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